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Jun 16, 2025
9:28 PM
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Introduction
1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride EDC HCl
is a widely used water-soluble carbodiimide reagent employed in peptide synthesis, protein conjugation, and organic transformations. Known for its efficiency and compatibility with aqueous environments, EDC HCl plays a crucial role in amide bond formation, especially in zero-length crosslinking reactions.
Chemical Identity
Chemical Name: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride
Abbreviation: EDC HCl
Molecular Formula: C8H17N3·HCl
Molecular Weight: 191.70 g/mol
CAS Number: 25952-53-8
Appearance: White to off-white crystalline powder
Solubility: Highly soluble in water and polar organic solvents (DMF, DMSO)
Key Applications of EDC HCl
1. Peptide Synthesis
EDC HCl is extensively used to form amide bonds between carboxyl and amine groups in peptide coupling, particularly in solution-phase and solid-phase synthesis.
2. Protein and Antibody Crosslinking
Acts as a zero-length crosslinker in bioconjugation, enabling direct coupling of proteins or peptides to other biomolecules or surfaces.
3. DNA and RNA Labeling
EDC HCl activates carboxyl-modified oligonucleotides, allowing for labeling and immobilization on various matrices.
4. Biomaterials Functionalization
Widely used to modify surfaces of hydrogels, nanoparticles, and polymer scaffolds by coupling bioactive ligands or peptides.
5. Carboxyl Activation in Organic Chemistry
Ideal for coupling reactions in the synthesis of esters, amides, and other carboxylic acid derivatives.
Advantages of EDC HCl
Water-soluble and compatible with biological systems
Produces minimal by-products (urea derivatives)
Efficient at neutral to slightly acidic pH (4.5–7.5)
Does not introduce spacer atoms (zero-length)
Stable and easy to handle under ambient conditions
Handling and Storage
Store in a cool, dry place, away from moisture
Use with gloves and eye protection in a fume hood
Avoid prolonged exposure to air or moisture to maintain stability
Dispose of waste following local chemical safety guidelines
Packaging and Availability
Available in multiple pack sizes:
Laboratory Scale: 1g, 5g, 10g, 25g
Bulk Supply: 100g, 500g, 1kg, 5kg, 25kg fiber drums
Each batch is accompanied by a Certificate of Analysis (COA) and Material Safety Data Sheet (MSDS).
Conclusion
EDC HCl is a high-efficiency coupling reagent used in both research and industrial applications. Its versatility in bioconjugation, peptide synthesis, and surface modification makes it an indispensable tool in life sciences, biotechnology, and pharmaceutical development.
Looking to source EDC HCl in India, Italy, or globally? Contact us for bulk quotes, documentation, and reliable logistics support tailored to your needs.
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